Congratulations to Chenggang Liu on publishing this study in Plant Physiology. In this work, the results of a hyl1 suppressor screen are reported: several novel, dominant DCL1 alleles with mutations either in the helicase domain or in the RNaseIIIa domain. These alleles rescue hyl1 phenotypes but do not fix the microRNA processing accuracy defects typical of the hyl1 null mutant. In vitro, these alleles cleave pri-miRNAs with enhanced kinetics. In addition, a requirement for the DCL1 helicase domain for accurate processing of select pri-miRNAs is found.
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Michael Axtell, lab PI